Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663

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Retinoic acid induces HL-60 cell differentiation via the upregulation of miR-663

BACKGROUND Differentiation of the acute myeloid leukemia (AML) cell line HL-60 can be induced by all trans-retinoic acid (ATRA); however, the mechanism regulating this process has not been fully characterized. METHODS Using bioinformatics and in vitro experiments, we identified the microRNA gene expression profile of HL-60 cells during ATRA induced granulocytic differentiation. RESULTS Six ...

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Retinoic acid induced differentiation and commitment in HL-60 cells.

Human leukemic HL-60 cells are an established model for studies of differentiation induction. Retinoic acid (RA), 2 x 10(-6) M, was used to induce terminal differentiation, assayed as nitroblue tetrazol reduction (NBT) and expression of monocytic surface antigens, which were detected by monoclonal antibody Leu M3. In addition, transferrin receptor expression and the number of S + G2 + M-phase c...

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Novel retinoic acid, 9-cis retinoic acid, in combination with all-trans retinoic acid is an effective inducer of differentiation of retinoic acid-resistant HL-60 cells.

Recent studies have shown that a high proportion of patients with acute promyelocytic leukemia (APL) achieve complete remission after treatment with all-trans retinoic acid (RA). Nevertheless, despite an initial good response, most patients that received continuous treatment with all-trans RA relapse and develop RA-resistant disease. The 9-cis RA is a high-affinity ligand for retinoid X recepto...

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ژورنال

عنوان ژورنال: Journal of Hematology & Oncology

سال: 2011

ISSN: 1756-8722

DOI: 10.1186/1756-8722-4-20